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French Open Champion Svetlana Kuznetsova was sent back to the drawing board, with her run coming to a halt at the AEGON International in Eastbourne.
The Russian was playing her first match since collecting her second Grand Slam title 10 days ago but was humbled by Canadian Aleksandra Wozniak.
The No.2 seed Kuznetsova only managed to win three games as she conceded defeat to the World No. 23.
Serbian Jelena Jankovic also continued her string of losses as she too exited in the opening round.
Jankovic, who started the year as No.1, has struggled for results in 2009 and admitted that she is lacking in confidence.